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| Thesis Name： |
Study of protein binding of amlodipine and levamlo |
| Usage： |
Study of protein binding of amlodipine and levamlo |
| Keyword： |
123 |
| Remarks： |
Study of protein binding of amlodipine and levamlodipine in human plasma albumin and bovine serum albumin solution by high performance capillary electrophoresis frontal analysis
The protein binding constants, binding sites and binding rate of Amlodipine and Levamlodipine in Human Plasma Albumin and Bovine Serum Albumin Solution were studied respectively by High performance capillary electrophoresis frontal analysis (HPCE/FA). The experiments were carried out by determining unbound drug concentration in drug-protein equilibrium solution in an uncoated fused silica capillary (40 cm × 50 mm i.d.; effective length 32 cm) for capillary electrophoresis under the following conditions: buffer, sodium phosphate solution (pH 7.4, ionic strength 0.17); UV detector, wavelength 214 nm; hydrostatic injection mode and the injection time 100 s, run voltage 10 kV. The unbound drug concentration was calculated from the plateau height of the profile with good linear relations, r>0.999 for both Amlodipine (N=6) and Levamlodipine(N=6), respectively. The determination of binding parameters was performed in accordance with Scatchard and Klotz equation. The reproducibility of the datum determined by HPCE/FA was confirmed by RSD (RSD < 0.39 %). The capillary electrophoresis frontal analysis provides an easy, accurate method for pharmacokinetics and pharmacodynamics study of the drug.
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| Original file： |
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Promulgator Detail |
| Full name: |
hichem |
| Country: |
CN |
| City: |
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| Company Name: |
hichem |
| Homepage: |
http://www.teloon.com |
| E-mail: |
reply |
| Phone: |
86-411-82819585 |
| Fax: |
86-411-82819612 |
| Company Address: |
Renming Road, Dalian city, Lianning pro,china |
| Zip/Postal Code: |
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| Introduction: |
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